Biosensors, Facilitated Diffusion in
Membranes, Restricted Diffusion in Membranes, Transport Processes in Tissues,
Pharmacokinetics, Immobilized Enzymes, Bioimaging.
Optical Semi-noninvasive Glucose Sensor
Schematics illustrating the principles of the
fluorescence affinity hollow fiber sensor. In the absence of glucose,
fluorochromelabeled Concanavalin A is bound to fixed glucose residues inside porous
beads (left hand). The beads are colored with dyes that prevent the excitation
light from penetrating into them and inducing Con A to fluoresce, thus keeping
the fluorescence emission at 520 nm. After diffusion of glucose through the
hollow fiber membrane (molecular weight cutoff, 10 kDa), Con A is displaced
from the beads and diffuses out of them, and hereby fluorochrome-labeled Con A becomes
exposed to excitation light resulting in a strong increase in fluorescence
(right hand).
Design of a GIP (glucose indicator protein) for
sensing glucose based on FRET. (a) Diagram of the GIP structure showing how FRET
between two GFPs can measure glucose concentration. The GBP adopts an “open”
form in the presence of the glucose, which triggers a conformation change,
causing two GFPs apart from the center of GBP leading to the change in FRET.
The (b)
represents one molecule of the glucose
bound onto the binding cleft of the GBP.
Transmission Plasmon Sensor for Biomolecules
(a) SEM image of nanoholes in an optically thin Au
film. The holes are 60 nm in diameter, and the Au film is 20 nm thick. (b) Transmission
extinction spectra of Au nanoholes on a glass slide. The spectra were taken
after the surfaces of Au and glass were (i) cleaned in a mixture of ammonia and
hydrogen peroxide, (ii) modified with SAM coatings of DT and OTS, and (iii)
further modified by BSA adsorption. The resonance peak shifted about 1.5 and 7
nm to the right after steps (ii) and (iii), respectively.
Hindered Diffusion in Nanoporous Membrane
Pharmacokinetics of Peritoneal Transport
Biomaterials Testing
Annular flow
device for determination of the
effect of shear on thrombus formation on
different
materials.
Separation of gases by facilitated diffusion in
liquid membranes